| Molecular |
| 1 |
Basics of molecular biology |
- Reagent preparation
- Genomic DNA isolation from plant
- Plasmid DNA isolation
- Agarose gel electrophoresis
|
4 days |
| 2 |
Advance molecular biology I |
- Plasmid DNA isolation
- DNA purity determination
- Competent cell preparations
- Transformation
- Blue white screening
|
5-6 days |
| 3 |
Advance molecular biology II |
|
2 days |
- Southern hybridization
- SDS- PAGE
- Native – PAGE
|
3-4 days |
| Microbiology |
| 1 |
Basics of microbiology |
- Good laboratory practices
- Media preparation
- Plate Pouring
- Streaking
- Spreading
- Gram’s staining
- Colony characteristics
|
3 days |
| 2 |
Advance microbiology I |
- Good laboratory practices
- Isolation of microbes
- Identification of Microbes by
Biochemical Characterization by
IMVIC Test
- Long term preservation of microbes
|
3 days |
| 3 |
Advance microbiology II |
- Good laboratory practices
- Isolation of microbes from different sources.
- Identification of Microbes by Biochemical Characterization.
- IMViC series Test.
- Starch Hydrolysis Test.
- Casein Hydrolysis Test.
- Antibiotic sensitivity test.
- Minimum Inhibitory concentration (MIC) Test.
|
4-5 days |
| Enzymology |
| 1 |
Basics of enzymology |
- Isolation of enzyme producing microorganism from suitable substrate.
- Pure culture Preparation.
- Screening of purified cultures for Enzyme activity.
|
4 days |
| 2 |
Advance enzymology |
- Isolation of enzyme producing microorganism from suitable substrate.
- Pure culture Preparation.
- Screening of purified cultures for Enzyme activity.
- Fermentation.
- Extraction of Crude Enzyme.
- Precipitation of Enzyme.
|
4-5 days |
- Total protein estimation
- Bradford’s Method.
- Lowry’s Method
- SDS PAGE.
- Native PAGE.
|
3-4 days |
| Immunology |
| 1 |
Basics of immunology |
- WIDAL test
- Blood grouping
- Double diffusion test
- Radial immunodiffusion
|
2 days |
| 2 |
Advance immunology |
- Sandwich ELISA
- Rocket immunoelectrophoresis
|
4 days |
| Animal cell culture |
| 1 |
Basics techniques |
- Viable cell count by haemocytometer and TALI cytometer
- MTT assay
|
3 days |
| 2 |
Culture techniques |
- Revival of cell line
- Cell line maintenance
- Trypsinization
- Subculture
- Cell count
- Cell line preservation
|
4-5 days |
| Analytical techniques |
| 1 |
HPLC |
- System operation
- Mobile phase preparation
- Sample injection
- Sample injection
- Data analysis
|
2 days |
| 2 |
Gas chromatography |
- System operation
- Sample preparation
- Sample injection
- Data analysis
|
2 days |
| 3 |
Ion exchange chromatography |
- Reagent preparation
- Column packing
- Purification of enzyme
- Estimation of enzyme activity and calculation
|
3 days |
